Last data update: May 13, 2024. (Total: 46773 publications since 2009)
Records 1-4 (of 4 Records) |
Query Trace: Bessoff K[original query] |
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Avian hosts of West Nile virus in Puerto Rico
Komar N , Bessoff K , Diaz A , Amador M , Young G , Seda R , Perez T , Hunsperger E . Vector Borne Zoonotic Dis 2011 12 (1) 47-54 West Nile virus (WNV) ecology in neotropical ecosystems is poorly understood, and vertebrate hosts responsible for infecting mosquitoes remain unidentified throughout the Caribbean Basin. After a period of intense WNV transmission among sentinel chickens near Ceiba, Puerto Rico, we measured abundance of resident birds and species-specific prevalence of WNV infection. Taking the product of these measures indicates the relative number of WNV infections by species. Greater Antillean grackle (Quiscalus niger) accounted for the most WNV infections among birds in our 100-km(2) study site. In urban habitats, the house sparrow (Passer domesticus) was frequently infected. Immature birds less than one year of age were more likely to have detectable WNV-reactive antibodies than older birds of the same species. |
Dengue virus seroprevalence among febrile patients in Bamako, Mali: results of a 2006 surveillance study
Phoutrides EK , Coulibaly MB , George CM , Sacko A , Traore S , Bessoff K , Wiley MR , Kolivras KN , Adelman Z , Traore M , Hunsperger EA . Vector Borne Zoonotic Dis 2011 11 (11) 1479-85 BACKGROUND: Dengue viruses (DENV) are endemic in over 100 countries worldwide, and annually 50 to 100 million people are infected by one of the four DENV serotypes, whereas over 2.5 billion people are at risk for infection. West African countries lack the surveillance to determine the true incidence of dengue; hence, this disease is likely significantly underestimated. In Mali, approximately 14 million people are potentially at risk of acquiring a dengue infection. METHODS AND FINDINGS: A serosurvey for DENV was conducted on 95 human serum samples obtained from the Institute National de Recherche en Sante Publique in 2006. DENV-specific IgM and IgG enzyme-linked immunosorbent assays were performed on all samples, and a subset was tested using the plaque-reduction neutralization test against the DENV and yellow fever virus (YFV). Samples collected during the acute infection (0-5 days postonset of symptoms) were tested for dengue NS1 antigen and reverse-transcriptase polymerase chain reaction for Flaviviruses, Alphaviruses, and Bunyaviruses RNA. A total of 87 (93%) of samples were positive for anti-DENV IgG antibodies. Of a subset of 13 IgG positive samples, 2 samples neutralized monotypically against DENV-1 and -2, whereas 3 others neutralized broadly against YFV and multiple DENV. Although no polymerase chain reaction positives were found, DENV NS1 was detected in 1 of the 20 acute samples tested. CONCLUSIONS: Of the 93 human serum samples tested, the dengue prevalence based on dengue IgG enzyme-linked immunosorbent assay results was 93%. Three DENV specific positive samples and two YFV positives were identified by plaque-reduction neutralization test. Finally, one sample tested positive for dengue NS1, thus suggestive of an acute infection within 14 days of obtaining the sample from the patient. Based on these serological data from this study, YFV and DENV appear to be co-circulating in Mali. |
Defining the utility of a commercial NS1 antigen capture kit as a dengue virus (DENV) diagnostic tool
Bessoff K , Phoutrides E , Delorey M , Acosta LN , Hunsperger E . Clin Vaccine Immunol 2010 17 (6) 949-53 Annually, over 2.5 billion people are at risk for infection by dengue virus (DENV), while between 50 and 100 million people contract the disease. There is an urgent need for alternative diagnostic tools that can detect DENV during acute infection. Recent studies have shown that dengue nonstructural protein 1 (NS1) is detectable in the blood as early as the onset of symptoms and persist well into the convalescent phase of the infection. We evaluated the utility of the BioRad Platelia Dengue NS1 Antigen Capture(R) kit in combination with real time reverse transcriptase polymerase chain reaction (RT-PCR) and IgM antibody capture enzyme linked immunosorbent assay (MAC ELISA) for refining a new diagnostic algorithm for the diagnosis of an acute or convalescent DENV from a single clinical sample. We tested the BioRad kit in three panels of sera. These panels were designed to evaluate the sensitivity of the NS1 kit in (1) early convalescent samples, (2) PCR false negative acute samples, and (3) confirmed secondary dengue infections with IgM negative convalescent samples. Results show that the NS1 can be detected in 22% serum collected after 10 days post onset of illness and in 22% of samples that did not elicit an IgM response. Additionally, NS1 was detected in 37% of the PCR false negative acute samples tested suggesting that NS1 detection may be valuable in increasing the sensitivity of current acute phase diagnostics. These results will improve diagnosis in a single acute or early convalescent sample for disease surveillance and clinical diagnosis. |
West Nile virus from blood donors, vertebrates, and mosquitoes, Puerto Rico, 2007
Hunsperger EA , McElroy KL , Bessoff K , Colon C , Barrera R , Munoz-Jordan JL . Emerg Infect Dis 2009 15 (8) 1298-300 West Nile virus (WNV) was isolated from a human blood donor, a dead falcon, and mosquitoes in Puerto Rico in 2007. Phylogenetic analysis of the 4 isolates suggests a recent introduction of lineage I WNV that is closely related to WNV currently circulating in North America. |
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